As global seed trade, variety breeding, and seed quality regulation continue to advance, seed laboratories are increasingly developing toward standardization, digitalization, and automation. Whether serving the Southeast Asian market, the Russian market, or research breeding and commercial seed testing in Europe and North America, laboratory construction must be based on internationally recognized standards to ensure accurate and globally accepted testing results.
Among these standards, the ISTA Rules established by the International Seed Testing Association have become an important foundation for global seed quality testing and a key reference system for building standardized seed laboratories.
In the ISTA (International Seed Testing Association) standard system, Sampling is one of the most critical preliminary steps in seed testing, because all subsequent results—such as purity, germination, moisture, and health testing—depend on whether the sample is representative.
In ISTA Purity Analysis, the core principle is to accurately separate and determine the proportion of:
Used to evaluate seed size, fullness, seeding rate calculation, and varietal characteristics.
It represents the average weight of 1,000 seeds, usually expressed in grams (g).
Seed moisture content directly affects storage safety and germination ability.
1.Oven Method — Standard Arbitration / Reference Method
2.Moisture Meter Method — Rapid Moisture Detection
Germination testing is one of the core testing items in seed laboratories.
Using a Vacuum Seed Counter & Seeder, Germination Board, and Suction Pen for sowing and seed placement → Place seeds into germination boxes and germination paper for incubation → Observe and evaluate germination in a Climate Chamber (light, temperature, humidity controlled) or Illuminated Incubator (light and temperature controlled).
Vigour testing is used to evaluate seed germination performance and field emergence potential under stress conditions.
| Test | Purpose | Equipment | Typical Crops |
|---|---|---|---|
| Accelerated Aging Test | Storage life and anti-aging ability | Seed Aging Chamber | Soybean, Corn |
| Cold Test | Low-temperature emergence ability and early sowing adaptability | Low-temperature Plant Incubator | Corn |
| Conductivity Test | Membrane integrity and damage level | Conductivity Meter | Pea, Soybean |
Seed health testing is used to detect pathogens such as fungi, bacteria, and viruses.
Culture Method — Seeds are incubated under suitable conditions to allow pathogens to grow for observation and identification.
Microscopic Observation — Direct observation of pathogen morphology on seed surfaces, inside seeds, or after culturing.
PCR Molecular Detection — Extraction of pathogen DNA/RNA followed by amplification using specific primers.
GMO testing has become an important testing item in international trade.
Used for rapid reading of GMO test strips or on-site rapid screening.
Corn, Soybean, Cotton, Rapeseed
Port quarantine, grain collection stations, incoming material screening in seed companies.
Immunochromatographic detection of GMO-expressed proteins.
Commonly used for detecting glyphosate-tolerance protein expression, such as Roundup Ready series.
Soybean, Corn, Rapeseed
Bt insect-resistant proteins including:
Suitable for corn, cotton, and other crops.
The X-ray test is a non-destructive testing method used to rapidly observe the internal structure of seeds.
With the development of smart agriculture and digital breeding, automated seed phenotyping is becoming an important direction in the modern seed industry.


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